Hits:Updated:2019-08-28 15:08:06【Print】
In recent years, scholars from various countries have carried out extensive chemical and pharmacological studies on different varieties of sea cucumbers. We extracted and extracted sea cucumber from the freshly collected viscera of sea cucumber, which proved to have anti-tumor antifungal effect. It is clinically used to treat dermatophytosis and achieve better curative effect. Extracting and separating polysaccharides from the dried body wall of Jade Sea Cucumber has the functions of improving immune function, anti-tumor and anti-hemagglutination. The results of the medicinal research on the internal organs of jade foot sea cucumber are reported below.
First, the source of materials
Jade foot sea cucumber is collected in the waters near the Wotou Pearl Farm in Huiyang, Guangdong Province. The water depth is 1-2 meters. The live sea cucumbers are kept in the sea for 1 day to remove a large amount of sediment and then dissected into body wall and internal organs. (including Guevance), the body wall is dried, and the internal organs are fixed with 70-80% ethanol.
Second, the extraction and separation of sea cucumber
The viscera of the sea cucumber that has been removed from the sediment will be chopped (10,000 rpm / 3 minutes) with a high-speed masher, and then immersed in 70-80% ethanol for 3 times, each time for about 1 week. Ethanol is recovered by pressure to extract (brown red), and the extract is repeatedly dissolved in methanol to remove insoluble matter. The methanol component is recovered under reduced pressure in a methanol extract, dissolved in water, and the insoluble matter is filtered off. After extracting 3 times, the degreased aqueous solution is extracted several times with n-butanol, and the n-butanol layer is combined, dried with anhydrous sodium sulfate, filtered to remove sodium sulfate, and the n-butanol is recovered to dryness under reduced pressure to obtain a pale yellow powder. Prime. The substance has obvious acetic anhydride-concentrated sulfuric acid reaction and α-nonylphenol reaction, and belongs to the group of saponins.
The sea cucumber was separated by silica gel column chromatography, and the particle size of the silica gel was 100-200 mesh. The eluent was collected in different proportions of chloroform:methanol:water, and 1 part was collected every 50 ml. The supernatant was examined with a silica gel layer. Chloroform: methanol = 2:1, the developer was 5% phosphomolybdic acid ethanol solution, and the same spots were combined. The solvent was recovered to obtain sea cucumbers I, IV, V, and VI.
Third, preliminary identification of sea cucumber I
The sea cucumber saponin isolated from the viscera of Jade foot sea cucumber has the highest content of sea cucumber I. The preliminary chemical identification of sea cucumber I has proved that sea cucumber I belongs to triterpenoid saponin, and after hydrolysis, it is separated into monomeric unit. , infrared, ultraviolet, mass spectrometry and H1-NMR measurements, consistent with the known aglycon 22,25-oxidoholothurinogenin.
Using the standard sample of sea cucumber B as the control, the Rf value of the silica gel thin layer chromatography of sea cucumber I was the same as that of the control, and the infrared spectrum also had the absorption of γ-lacto absorption band (1750 cm-1) and 甙 (3389 cm-1). , 1060cm-1), consistent with the infrared absorption of sea cucumber B reported in the literature, it is preliminarily believed that the chemical structure of sea cucumber I is the same as that of sea cucumber B.
Effects of sea cucumber on cervical epithelial cancer cells cultured in vitro
Sea cucumber I had significant inhibitory effect on cervical epithelial cancer cells. The inhibition rate of sea cucumber pigment I (10 μg/ml) was 100%, and the positive control drug Changchunxin (10 μg/ml) inhibition rate was 87.3%. The inhibitory effect of sea cucumber I on Hela cells was stronger than that of Changchunxin.
The effect of sea cucumber I on normal human embryo lung cells was only 10.34% when the concentration was 0.1 mg/ml, while the inhibition rate of normal human embryo lung cells was 51.25% at the same concentration. The effect of sea cucumber I on normal cytotoxicity is less than that of Changchun Xinyu.
5. Antitumor effect of sea cucumber on mouse S-180
The dose of sea cucumber extract was 50 mg/kg, and it was intraperitoneally administered. The tumor inhibition rate of mouse S-100 was 46.7%.
The dose of sea cucumber I was 2 mg/kg, and it was administered intraperitoneally. The tumor inhibition rate of mouse S-180 was 57.7%.
Six, the antifungal effect of sea cucumber
Sea cucumber has a strong antifungal effect. When the concentration is 10-40μg/ml, it can inhibit the growth of Astragalus, Hairy bacillus and Cryptococcus. Try to treat 113 cases of dermatophytes, and get better results.
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